High Throughput Sequencing Facility
The GRCF HTS Center was established to provide the research community at Johns Hopkins University with access to ‘next generation’ sequencing platforms. We currently feature an Illumina Genome Analyzer II and HiSeq2000, which have the capability to generate sequences with high accuracy at an extremely low cost per base. Using a sequencing-by-synthesis chemistry, billions of bases are produced with each run at a cost of less than 1% of capillary based methods.
The GAII and HiSeq offer some exciting capabilities. Among them are resequencing, de novo sequencing, SNP discovery, transcript counting, small RNA sequencing, ChIP-seq, and DNA Methylation sequencing.
We now offer exome sequencing using Agilent’s Sureselect platform, and whole genome sequencing through Complete Genomics.
Both single read and paired-end sequencing with read lengths of up to 75 bp are offered.
